Cod Uracil-DNA Glycosylase (Cod UNG)

Descriptive papers

Lanes O., et al. (2000): Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua). Comparative Biochemistry and Physiology – Part B: Biochemistry & Molecular Biology.. 127, 399-410.

Lanes O., et al. (2002): Identification, cloning, and expression of uracil-DNA glycosylase from Atlantic cod (Gadus morhua): characterization and homology modeling of the cold-active catalytic domain. Extremophiles 6, 73-86.

Leiros I., et al. (2001): Crystallization and preliminary X-ray diffraction analysis of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua). Acta Crystallographica. D57, 1706-1708.

Leiros I., et al. (2003): The crystal structure of Uracil-DNA N-glycosylase from Atlantic cod (Gadus morhua) reveals cold-adapted features. Acta Crystallographica. D59, 1357-1365. 2003.

Moe E., Leiros I., Riise E.K., Olufsen M. et. al (2004):  Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNAN-glycosylase (UNG) from Atlantic cod (Gadus morhua). Journal of molecular Biology Nov 5: 1221-1230. Vol 343: Issue 5.

Olufsen M., et al (2005): Increased flexibility as a strategy for cold adaptation.
The Journal of Biological Chemistry, 280,18042-18048.

Olufsen M., Brandsdal B.O., Smalås A.O. (2007): Comparative unfolding studies of psychrophilic and mesophilic uracil DNA glycosylase: MD simulations show reduced thermal stability of the cold-adapted enzyme. Journal of Molecular Graphics and Modelling. Vol 26, Iss 1: 124-134.

Papers showing use of Uracil-DNA glycosylase

Briggs A.W., et al. (2009): Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA. Nucleic Acids Research. doi:10.1093/nar/gkp1163

Blair P.L., et al. (2002): Transcripts of developmentally regulated Plasmodium falciparum genes quantified by real-time RT-PCR. Nucleic Acids Research. 30(10): 2224-2231.

Champlot Sophie, Camille Berthelot, Mélanie Pruvost, E. Andrew Bennett, Thierry Grange, and Eva-Maria Geigl. (2010): An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications. PLoS ONE 5(9): e13042. doi:10.1371/journal.pone.0013042

Collis S.J., et al. (2002): Development of a novel rapid assay to assess the fidelity of DNA double-strand-break repair in human tumour cells. Nucleic Acids Research. 30(2): e1.

Connolly J., et al. (1999): Mutational analysis of the engrailed homeodomain recognition helix by phage display. Nucleic Acids Research. 27(4): 1182-1189.

Epstein U.J., et al. (1993): A novel method employing UNG to avoid carry-over contamination in RNA- PCR. Nucleic Acids Research. 21(16): 3917-3918.

Kwok S., Higuchi R. (1989):
Avoiding false positives with PCR.
Nature 339, 237 – 238

Jacobsen N., et al. (2004): Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture. Nucleic Acids Research. 32(7): e64.

Kleiboeker S.B. (2005): Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR. Virology Journal 2, 29

Leblanc J.J., Pettipas J., Campbell S.J., Davidson R.J., Hatchette T.F. (2008): Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes. J Virol Methods.  Jul;151(1):158-60. Epub 2008 May 12.

Longo M.C., et al. (1990): Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions Gene 93, 125-128.

Pruvost M. et al. (2005): Minimizing DNA contamination by using UNG-coupled quantitative real-time PCR on degraded DNA samples: application to ancient DNA studies. BioTechniques 38:569-575.

Schwarz G., et al. (2004): Determination of detection and quantification limits for SNP allele frequency estimation in DNA pools using real time PCR. Nucleic Acids Research. 32(3): e24-.

Wu X., Wang Z. (1999): Relationships between yeast Rad27 and Apn1 in response to apurinic/apyrimidinic (AP) sites in DNA Nucleic Acids Research. 27(4): 956-962.

Zeschnigk M., et al. (2004): A novel real-time PCR assay for quantitative analysis of methylated alleles (QAMA): analysis of the retinoblastoma locus. Nucleic Acids Research. 32(16): e125-.