Cod Uracil-DNA Glycosylase (Cod UNG)
Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic cod is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.
Main advantages with Cod UNG
- Heat-labile
- Completely and irreversibly inactivated at 50°C
- Use of Cod UNG makes contamination control possible in RT-PCR
- Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible
- High purity enzyme, tested free of contaminating nucleases
- No detectable content of E. coli DNA in enzyme product
Specifications
Unit definition: One Unit will liberate 1 nmol Uracil from Uracil-containing DNA per hour at 37°C.
Specific activity: >500 000 Units/mg.
Purity: Purified to apparent homogeneity by SDS-PAGE. No nuclease activity is detected.
Concentration: Minimum 1 000 Units/ml.
Properties:
Stability: Minimum shelf life at -20°C is 2 years. In practice we find that storage at 4°C is possible for at least 6 months. The enzyme activity is also preserved upon multiple freeze-thaw cycles.
