Main advantages with Cod UNG

• Heat-labile
• Completely and irreversibly inactivated at 50°C
• Use of Cod UNG makes contamination control possible in RT-PCR
• Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible
• High purity enzyme, tested free of contaminating nucleases
• No detectable content of E. coli DNA in enzyme product

Specifications

Unit definition: One Unit will liberate 1 nmol Uracil from Uracil-containing DNA per hour at 37°C.
Specific activity: >500 000 Units/mg.
Purity: Purified to apparent homogeneity by SDS-PAGE. No nuclease activity is detected.
Concentration: Minimum 1 000 Units/ml.

Properties:
Stability: Minimum shelf life at -20°C is 2 years. In practice we find that storage at 4°C is possible for at least 6 months. The enzyme activity is also preserved upon multiple freeze-thaw cycles.

Applications
Publications