ArcticZymes » Shrimp Alkaline Phosphatase (SAP) http://www.arcticzymes.com Heat-labile enzymes - for your convenience Tue, 24 Jan 2012 15:26:06 +0000 en hourly 1 Shrimp Alkaline Phosphatase (SAP) http://www.arcticzymes.com/products/shrimp-alkaline-phosphatase/shrimp-alkaline-phosphatase-sap/ http://www.arcticzymes.com/products/shrimp-alkaline-phosphatase/shrimp-alkaline-phosphatase-sap/#comments Thu, 04 Feb 2010 10:44:37 +0000 admin http://www.marinebiochem.com/?p=65 Shrimp Alkaline Phosphatase (SAP) has become one of today’s most-selling DNA modifying enzymes due to the added convenience through a complete heat inactivation. While other alkaline phosphatases (from E. coli or Calf intestine) must be removed by extraction procedures, SAP is completely inactivated after 15 minutes at 65°C. SAP works well in common buffers without the requirement for other additions.

Main advantages with SAP

  • Very high specific activity
  • 100% heat-inactivated at 65°C
  • Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
  • May be added directly to restriction enzyme digests
  • No vector purification necessary
  • Requires no supplemental zinc or other additives for activity
  • Works directly in many different buffers
  • Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis
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